SARS-CoV-2 NSP10 Protein

NSP10 is a major regulator of coronavirus replicase function. NSP10 contains two zinc fingers and binds and stimulates both NSP14 and NSP16 activities. Researchers has found that the nsp10 surface that interacts with nsp14 and nsp16 and possibly other subunits of the viral replication complex may be a target for the development of antiviral compounds against pathogenic coronaviruses. SARS-CoV-2 NSP10 Protein is expressed in E. coli expression system. The predicted molecular weight is 15 kDa and the accession number is YP_009725306.1.

SARS-CoV-2 NSP10 Protein

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 160,500

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説明

Species	SARS-CoV-2
Expression Host	E. coli
Tag	Tag Free
Accession Number	YP_009725306.1
Construction	The SARS-CoV-2 (2019-nCoV) NSP10 (YP_009725306.1) (Ala1-Gln139) was expressed with two amino acids (GP) at the N-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE.
分子量	15 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	NSP10 is a major regulator of coronavirus replicase function. NSP10 contains two zinc fingers and binds and stimulates both NSP14 and NSP16 activities. Researchers has found that the nsp10 surface that interacts with nsp14 and nsp16 and possibly other subunits of the viral replication complex may be a target for the development of antiviral compounds against pathogenic coronaviruses.

参考文献

1. Mickaël Bouvet,et al.Coronavirus Nsp10, a Critical Co-factor for Activation of Multiple Replicative Enzymes.THE JOURNAL OF BIOLOGICAL CHEMISTRY.2014Yuanyuan Ma,et al.Structural basis and functional analysis of the SARS coronavirus nsp14-nsp10 complex. Proc Natl Acad Sci USA. 2015Etienne Decroly,et al.Crystal structure and functional analysis of the SARS-coronavirus RNA cap 2'-O-methyltransferase nsp10/nsp16 complex. PLoS Pathog.2011

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SARS-CoV-2 NSP10 Protein

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