SARS-CoV-2 NSP7 Protein

NSP7 is conserved within the coronaviridae. NSP7 is a component of the coronavirus replicase polyprotein to comprise a repilication complex. NSP7 has been shown to interact with NSP10 and NSP1 which indicate that NSP7 has a founction in coronavirus-specific RNA replication mechanisms.

SARS-CoV-2 NSP7 Protein

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 160,500

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説明	NSP7 is conserved within the coronaviridae. NSP7 is a component of the coronavirus replicase polyprotein to comprise a repilication complex. NSP7 has been shown to interact with NSP10 and NSP1 which indicate that NSP7 has a founction in coronavirus-specific RNA replication mechanisms.

Species	SARS-CoV-2
Expression Host	E. coli
Tag	Tag Free
Accession Number	YP_009725303.1
Construction	The SARS-CoV-2 (2019-nCoV) NSP7 Protein (YP_009725303.1) (Ser1-Gln83) was expressed with two amino acids (GP) at the N-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE.
分子量	9.4 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	NSP7 is conserved within the coronaviridae. NSP7 is a component of the coronavirus replicase polyprotein to comprise a repilication complex. NSP7 has been shown to interact with NSP10 and NSP1 which indicate that NSP7 has a founction in coronavirus-specific RNA replication mechanisms.

参考文献

1. Wolfgang Peti, et al.Structural Genomics of the Severe Acute Respiratory Syndrome Coronavirus: Nuclear Magnetic Resonance Structure of the Protein nsP7. 2. JOURNAL OF VIROLOGY.2005Yibei Xiao,et al.Nonstructural proteins 7 and 8 of feline coronavirus form a 2:1 heterotrimer that exhibits primer-independent RNA polymerase activity. J Virol. 2012

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SARS-CoV-2 NSP7 Protein

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