SARS-CoV-2 NSP8 Protein (Avi)

NSP8 is a nonstructural protein of coronavirus. NSP8 acts as a primase in RNA synthesis. NSP8 and NSP7 are essential co-factors of NSP12 (the catalytic subunit with RNA-dependent RNA polymerase activity) that can remarkably stimulate RdRp activity. The nsp12-nsp7-nsp8 subcomplex is defined as the minimal core component for mediating coronavirus RNA synthesis. SARS-CoV-2 NSP8 Protein (Avi) is expressed in E. coli expression system. The predicted molecular weight is 24.17 kDa and the accession number is YP_009725304.1.

SARS-CoV-2 NSP8 Protein (Avi)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 160,500

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説明

Species	SARS-CoV-2
Expression Host	E. coli
Tag	C-Avi
Accession Number	YP_009725304.1
Construction	The SARS-CoV-2 (2019-nCoV) NSP8 Protein (YP_009725304.1) (Ala1-Gln198) was expressed with a AVI tag at the C-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE.
分子量	24.17 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris 500 mM NaCl pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	NSP8 is a nonstructural protein of coronavirus. NSP8 acts as a primase in RNA synthesis. NSP8 and NSP7 are essential co-factors of NSP12 (the catalytic subunit with RNA-dependent RNA polymerase activity) that can remarkably stimulate RdRp activity. The nsp12-nsp7-nsp8 subcomplex is defined as the minimal core component for mediating coronavirus RNA synthesis.

参考文献

1. Qi Peng,et al.Structural and Biochemical Characterization of the nsp12-nsp7-nsp8 Core Polymerase Complex from SARS-CoV-2.Cell Reports.2020 2. Yan Gao,et al.Structure of the RNA-dependent RNA polymerase from COVID-19 virus.Science.2020 3. Purnima Kumar,et al.The nonstructural protein 8 (nsp8) of the SARS coronavirus interacts with its ORF6 accessory protein.Virology.2007

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SARS-CoV-2 NSP8 Protein (Avi)

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