SARS-CoV-2 (B.1.1.529 sublineage BA.2) Nucleocapsid Protein (His)

SARS-CoV-2 (B.1.1.529 sublineage BA.2) Nucleocapsid Protein (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 46.38 kDa and the accession number is YP_009724397.2.

SARS-CoV-2 (B.1.1.529 sublineage BA.2) Nucleocapsid Protein (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 160,500

バルクのお問い合わせ

説明	SARS-CoV-2 (B.1.1.529 sublineage BA.2) Nucleocapsid Protein (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 46.38 kDa and the accession number is YP_009724397.2.

Species	SARS-CoV-2
Expression Host	E. coli
Tag	N-His
Accession Number	YP_009724397.2
Construction	The SARS-CoV-2 (B.1.1.529 sublineage BA.2) Nucleocapsid (YP_009724397.2, with mutations P13L, ERS31-33 del, R203K, G204R, S413R) (Met1-Ala419) was expressed with a polyhistidine tag at the N-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.1.529 sublineage BA.2).
Protein Purity	> 90 % as determined by SDS-PAGE.
分子量	46.38 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM PB, 500 mM NaCl, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

参考文献

1. Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.Li W. et al., 2005, Science. 309 (5742): 1864-8.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

SARS-CoV-2 (B.1.1.529 sublineage BA.2) Nucleocapsid Protein (His)

参考文献

Calculator

技術サポート

Keywords