| 説明 |
SARS-CoV Nucleocapsid Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 47.5 kDa and the accession number is Q6S8E1. |
| Species |
SARS
|
| Expression Host |
Baculovirus Insect Cells
|
| Tag |
C-His
|
| Accession Number |
Q6S8E1
|
| 別名 |
coronavirus Nucleocapsid Protein, SARS, coronavirus NP Protein, SARS, novel coronavirus NP Protein, SARS, ncov NP Protein, SARS, novel coronavirus, cov np Protein, SARS, novel coronavirus Nucleocapsid Protein, SARS, coronavirus Nucleoprotein Protein, SARS
|
| Construction |
SARS-CoV (isolate:Tor2) nucleoprotein (NP_828858.1) (Met1-Ala422)
|
| Protein Purity |
> 80 % as determined by SDS-PAGE
|
| 分子量 |
47.5 kDa (predicted)
|
| Endotoxin |
< 1.0 EU/μg of the protein as determined by the LAL method.
|
| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
|
| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
|
| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
|
| Shipping |
In general, Lyophilized powders are shipping with blue ice.
|
| Research Background |
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
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参考文献
Calculator
Reconstitution Calculator
Recombinant Proteins Dilute Calculator
Specific Activity Calculator
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