SARS-CoV (strain WH20) Plpro/papain-like protease (His)

SARS-CoV (strain WH20) Plpro/papain-like protease (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 36.65 kDa and the accession number is AAX16193.1.

SARS-CoV (strain WH20) Plpro/papain-like protease (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 207,500

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説明	SARS-CoV (strain WH20) Plpro/papain-like protease (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 36.65 kDa and the accession number is AAX16193.1.

Species	SARS
Expression Host	E. coli
Tag	His
Accession Number	AAX16193.1
Construction	A DNA sequence encoding the SARS-CoV (strain WH20) papain-like protease (PLpro) (AAX16193.1) (Glu1541-Tyr1859) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE.
分子量	36.65 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from sterile 50 mM Tris, 0. 1 % Tween, pH 9. 0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses. Targeting PLpro with antiviral drugs may have an advantage in not only inhibiting viral replication but also inhibiting the dysregulation of signaling cascades in infected cells that may lead to cell death in surrounding, uninfected cells.

参考文献

1. Yahira M. Báez-Santos.The SARS-coronavirus papain-like protease: Structure, function and inhibition by designed antiviral compounds. Antiviral Research.2015

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SARS-CoV (strain WH20) Plpro/papain-like protease (His)

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