SDF-1 Protein, Mouse, Recombinant

SDF-1 Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 8.1 kDa and the accession number is P40224-1.

SDF-1 Protein, Mouse, Recombinant

パッケージサイズ	在庫状況	単価(税別)
10 μg	約5 days	￥ 38,000
100 μg	約5 days	￥ 142,000
1 mg	約5 days	￥ 897,500

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説明	SDF-1 Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 8.1 kDa and the accession number is P40224-1.

Species	Mouse
Expression Host	E. coli
Tag	Tag Free
Accession Number	P40224-1
別名	Scyb12, Tpar1, Pbsf, chemokine (C-X-C motif) ligand 12, Sdf1, Tlsf
Construction	The mature form of Mouse CXCL12 (P40224-1) (Lys22-Lys89) was expressed and purified with an initial Met.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	8.1 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50% acetonitrile, 1% TFA. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The human stromal cell-derived factor-1 (SDF1), also known as CXCL12, is a small (8 kDa) cytokine highly conserved chemotactic cytokine belonging to the large family of CXC chemokines. SDF1 is expressed in two isoforms from a single gene that encodes two splice variants, SDF1α and SDF1β, which are identical except for the four residues present in the C-terminus of SDF1β but absent from SDF1α. The chemokine CXCL12 [stromal cell-derived factor-1 (SDF-1)] binds primarily to CXC receptor 4 (CXCR4; CD184). The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription. CXCL12 and CXCR4 that have been widely characterized in peripheral tissues and delineate their main functions in the CNS. Extensive evidence supports CXCL12 as a key regulator for early development of the CNS. In the mature CNS, CXCL12 modulates neurotransmission, neurotoxicity and neuroglial interactions. CXCL12 has crucial roles in the formation of multiple organ systems during embryogenesis and in the regulation of bone marrow haematopoiesis and immune function in the postnatal organism. Although considered an important factor in normal bone metabolism, recent studies implicate CXCL12 in the pathogenesis of several diseases involving the skeleton, including rheumatoid arthritis and cancers that metastasize to bone. The CXCL12/CXCR4 axis is involved in tumor progression, angiogenesis, metastasis, and survival. Pathologically enhanced CXCL12 signaling may promote the formation of new vessels through recruiting circulating endothelial progenitor cells or directly enhancing the migration/growth of endothelial cells. Therefore, CXCL12 signaling represents an important mechanism that regulates brain tumor angiogenesis/vasculogenesis and may provide potential targets for anti-angiogenic therapy in malignant gliomas.

参考文献

1. Bleul, C.C. et al., 1996, Nature. 382: 829-833. 2. Sapede, D. et al., 2005, Proc. Natl. Acad. Sci. USA. 102: 1714-1718. 3. Delgado, M.B. et al., 2001, Eur. J. Immunol. 31: 699-707. 4. Orimo, A. et al., 2005, Cell. 121: 335-348. 5. Kryczek, I. et al., 2007, Am. J. Physiol. Cell. Physiol. 292: C987-995. 6. Bbalabanian, K. et al., 2005, J. Biol. Chem. 280: 35760-35766.

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SDF-1 Protein, Mouse, Recombinant

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