SEMA3A Protein, Mouse, Recombinant (His)

SEMA3A Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 61.6 kDa and the accession number is A0A803Z7F8.

SEMA3A Protein, Mouse, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 207,500

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生物学的情報	Testing in progress
説明	SEMA3A Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 61.6 kDa and the accession number is A0A803Z7F8.

Species	Mouse
Expression Host	HEK293 Cells
Tag	N-His
Accession Number	A0A803Z7F8
別名	SemD, Semad, Hsema-I, SEMA1, coll-1, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
Construction	Mouse SEMA3A (NP_001230001.1) (Lys26-Phe546)
Protein Purity	> 95 % as determined by SDS-PAGE.
分子量	61.6 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 150 mM NaCl, 100 mM Argine, 100 mM glutamic acid, pH 8.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Semaphorins are a family of secreted and cell-bound signaling molecules defined by the presence of a common 5 aa Sema domain. They are best characterized in relation to axon guidance during development of the nervous system. The functions of Semaphorins 3A (SEMA3A) are mediated primarily through binding to the Neuropilin-1 (Npn-1) and Plexin-A1 coreceptor complex. Neuropilins lack a signaling-competent cytoplasetmic domain and ensure semaphorin binding, whereas the transmembrane receptor plexin mediates the intracellular response. As the first identified vertebrate semaphorin, SEMA3A functions either as a chemorepulsive agent inhibiting axonal outgrowth, or as a chemoattractive agent stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Its overexpression is associated with schizophrenia which is seen in various human tumor cell lines, and aberrant release is associated with the progression of Alzheimer's disease

参考文献

1. Giordano,A. et al., 2003, J Neurocytol.32(4):345-352. 2. Good, P. F. et al., 2005, J. Neurochem.91(3): 716-736. 3. Gu, C. et al., 2005, Science.307(5707): 265–268. 4. Chadborn,N.H. et al., 2006, J Cell Sci.119(Pt 5):951-957. 5. Schmidt,E.F. et al., 2007, Adv Exp Med Biol.600:1-11.

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SEMA3A Protein, Mouse, Recombinant (His)

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