SEMA7A Protein, Human, Recombinant (His)

SEMA7A Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 69.5 kDa and the accession number is O75326-1.

SEMA7A Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 96,000
500 μg	約5 days	￥ 383,500
1 mg	約5 days	￥ 643,000

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説明	SEMA7A Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 69.5 kDa and the accession number is O75326-1.

Species	Human
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	O75326-1
別名	Semaphorin-L, SEMAK1, CD108, MGC126692, Sema7A, CD108MGC126696, Semaphorin-K1, SEMAL, JMH, CDw108, Semaphorin-7A, H-SEMA-K1, Sema L, H-Sema-L
Construction	Gln45-Ala648
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	69.5 kDa (predicted). Due to glycosylation, the protein migrates to 70-75 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Semaphorin7A (Sema7A) plays an important role in the immunoregulation of the brain.Sema7A is upregulated in the epileptic brain and plays a potential role in the regulation of seizure activity in PTZ-kindled epileptic rats, which may be related to neuroinflammation. Sema7A promotes the inflammatory cytokines TNF-α and IL-6 as well as the growth of mossy fibers through the ERK pathway, suggesting that Sema7A may promote seizures by increasing neuroinflammation and activating pathological neural circuits. Sema7A plays a critical role in epilepsy and could be a potential therapeutic target for this neurological disorder.

参考文献

1. Deng J, Xu T, Yang J, Zhang KM, Li Q, Yu XY, Li R, Fu J, Jiang Q, Ma JX, Chen YM. Sema7A, a brain immune regulator, regulates seizure activity in PTZ-kindled epileptic rats. CNS Neurosci Ther. 2020 Jan;26(1):101-116. doi: 10.1111/cns.1318Epub 2019 Jun 9. PMID: 31179640; PMCID: PMC6930824.

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SEMA7A Protein, Human, Recombinant (His)

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