SEMA4D Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 80.7 kDa and the accession number is AAH54500.1.
| 生物学的情報 |
Measured by its ability to bind mouse CD45 hFc in functional ELISA.
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| 説明 |
SEMA4D Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 80.7 kDa and the accession number is AAH54500.1. |
| Species |
Human
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| Expression Host |
HEK293 Cells
|
| Tag |
His
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| Accession Number |
AAH54500.1
|
| 別名 |
CD100, COLL4, C9orf164, semaphorin 4D, SEMAJ, coll-4, M-sema-G
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| Construction |
The Human SEMA4D (AAH54500.1) extracellular domain (Met 1-Arg 734) was expressed, with a polyhistidine tag at the C-terminus.
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| Protein Purity |
> 92 % as determined by SDS-PAGE
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| 分子量 |
80.7 kDa (predicted)
|
| Endotoxin |
< 1.0 EU/μg of the protein as determined by the LAL method.
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| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
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| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
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| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
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| Shipping |
In general, Lyophilized powders are shipping with blue ice.
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| Research Background |
Semaphorin 4D (SEMA4D or CD100) is a member of the semaphorin family of proteins and an important mediator of the movement and differentiation of multiple cell types, including those of the immune, vascular, and nervous systems. VEGF and SEMA4D had a positive correlation with the malignant degree of ovarian cancer, and SEMA4D can serve as an independent prognostic factor. SEMA4D was the first semaphorin described to have immune functions and serves important roles in T cell priming, antibody production, and cell-to-cell adhesion. Proteolytic cleavage of SEMA4D from the cell surface gives rise to a soluble fragment of SEMA4D (sSEMA4D). Similar to the transmembranal form, sSEMA4D is thought to have immunoregulatory properties.
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