Serum Albumin Protein, Human, Recombinant (His & Avi)

Human serum albumin (HSA), the most prominent protein in plasma, binds different classes of ligands at multiple sites. HSA provides a depot for many compounds, affects pharmacokinetics of many drugs, holds some ligands in a strained orientation providing their metabolic modification, renders potential toxins harmless transporting them to disposal sites, accounts for most of the antioxidant capacity of human serum, and acts as a NO-carrier. Serum Albumin Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 69.4 kDa and the accession number is P02768-1.

Serum Albumin Protein, Human, Recombinant (His & Avi)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 13,000
500 μg	約5 days	￥ 53,000
1 mg	約5 days	￥ 89,000

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生物学的情報

Human Serum Albumin, His Tag immobilized on CM5 Chip can bind Human FcRn, His Tag with an affinity constant of 0.728 μM as determined in SPR assay.

説明

Species	Human
Expression Host	HEK293 Cells
Tag	C-His-Avi
Accession Number	P02768-1
別名	Serum albumin, PRO0903, Albumin, PRO1341, ALB, ANALBA, FDAH, PRO0883
Construction	Asp25-Leu609
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	The protein has a predicted MW of 69.4 kDa. Due to glycosylation, the protein migrates to 69-70 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Human serum albumin (HSA), the most prominent protein in plasma, binds different classes of ligands at multiple sites. HSA provides a depot for many compounds, affects pharmacokinetics of many drugs, holds some ligands in a strained orientation providing their metabolic modification, renders potential toxins harmless transporting them to disposal sites, accounts for most of the antioxidant capacity of human serum, and acts as a NO-carrier.

参考文献

1. Ishima Y, Maruyama T. [Human Serum Albumin as Carrier in Drug Delivery Systems]. Yakugaku Zasshi. 2016;136(1):39-47. Japanese. doi: 10.1248/yakushi.15-00227-PMID: 26725666.

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Serum Albumin Protein, Human, Recombinant (His & Avi)

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