SFRP2 Protein, Human, Recombinant (His)

SFRP2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 32.4 kDa and the accession number is Q96HF1.

SFRP2 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 112,000
500 μg	約5 days	￥ 447,500
1 mg	約5 days	￥ 746,000

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説明	SFRP2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 32.4 kDa and the accession number is Q96HF1.

Species	Human
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	Q96HF1
別名	Sarp1, SARP-1, sFRP-2, AI851596, Sfrp2, Sdf5
Construction	Leu25-Cys295
Protein Purity	> 95% as determined by Tris-Bis PAGE
分子量	The protein has a predicted MW of 32.4 kDa. Due to glycosylation, the protein migrates to 34-38 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PBS, 100 mM L-arginine (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	As biomarkers, DNA methylation is used to detect colorectal cancer (CRC) and make assessment of CRC prognosis. The published findings showed the association between the methylation of SFRP1, SFRP2, and WIF1, located in the Wnt signaling pathway, and the prognosis of CRC were not consistent. SFRP1, SFRP2, and WIF1 were frequently hypermethylated in CRC tumor tissues. It was apparent that the promoter hypermethylation of SFRP2 and co-hypermethylation of SFRP1 and SFRP2 might be considered as independent prognostic predictors for survival advantage of postoperative CRC patients.

参考文献

1. Liu X, et al. DNA methylation of SFRP1, SFRP2, and WIF1 and prognosis of postoperative colorectal cancer patients. BMC Cancer. 2019 Dec 12;19(1):1212. doi: 10.1186/s12885-019-6436-0. PMID: 31830937; PMCID: PMC6909551.

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SFRP2 Protein, Human, Recombinant (His)

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