SFRP4 Protein, Human, Recombinant (His)

SFRP4 Protein, Human, Recombinant (His) is expressed in CHO mammalian cells with His tag. The predicted molecular weight is 39 kDa and the accession number is Q6FHJ7.

SFRP4 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 138,000

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説明	SFRP4 Protein, Human, Recombinant (His) is expressed in CHO mammalian cells with His tag. The predicted molecular weight is 39 kDa and the accession number is Q6FHJ7.

Species	Human
Expression Host	CHO Cells
Tag	C-His
Accession Number	Q6FHJ7
別名	secreted frizzled-related protein 4, FRPHE, FRP-4, sFRP-4
Construction	The Human SFRP4 (NP_003005.2) (Met1-VAL346) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE.
分子量	39 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	SFRP family consists of five secreted glycoproteins in humans acting as extracellular signaling ligands. Each is approximately 3 amino acids in length and contains a cysteine-rich domain (CRD) that shares 3-5% sequence homology with the CRD of Frizzled (Fz) receptors, a putative signal sequence, and a conserved hydrophilic carboxy-terminal domain. SFRPs act as soluble modulators of Wnt signaling, counteracting Wnt-induced effects at high concentrations and promoting them at lower concentrations. SFRPs are able to bind Wnt proteins and Fz receptors in the extracellular compartment. The interaction between SFRPs and Wnt proteins prevents the latter from binding the Fz receptors. The Wnt pathway plays a key role in embryonic development, cell differentiation and cell proliferation. SFRP4 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins called FZ domain and a NTR domain. Mouse SFRP4 is highly expressed in the ovary, and is localized to granulosa cells of periovulatory follicles and corpora lutea. It plays a critical role in placental development and implantation, and is also an important factor in the development of the decidual fibrinoid zone, and in trophoblast apoptosis.

参考文献

1. Abu-Jawdeh G.M., et al.,(1999), Differential expression of frpHE: a novel human stromal protein of the secreted frizzled gene family, during the endometrial cycle and malignancy. Lab. Invest. 79:439-447. 2. Berndt T., et al., (2003), Secreted frizzled-related protein 4 is a potent tumor-derived phosphaturic agent.J. Clin. Invest. 112:785-794. 3. Ota T., et al.,(2004), Complete sequencing and characterization of 21,243 full-length human cDNAs.Nat. Genet. 36:40-45.

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SFRP4 Protein, Human, Recombinant (His)

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