SIRP alpha Protein, Cynomolgus, Recombinant (His)

SIRP alpha Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 38.93 kDa and the accession number is XP_015313153.1.

SIRP alpha Protein, Cynomolgus, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
200 μg	在庫あり	￥ 134,000

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生物学的情報	Measured by its binding ability in a functional ELISA. Immobilized cynoSIRPAh at 10 μg/mL (100 μL/well) can bind CD47-Fc , the EC50 of CD47-Fc is 3.0-10.0 ng/mL.
説明	SIRP alpha Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 38.93 kDa and the accession number is XP_015313153.1.

Species	Cynomolgus
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	XP_015313153.1
別名	signal-regulatory protein α, signal-regulatory protein alpha, SIRP α
Construction	The cynomolgus SIRPA (XP_015313153.1) (Met1-Tyr372) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE.
分子量	38.93 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Tyrosine-protein phosphatase non-receptor type substrate 1, also known as SHP substrate 1, Inhibitory receptor SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Macrophage fusion receptor, CD172 antigen-like family member A, SIRPA and CD172a, is a single-pass type I membrane protein which contains two Ig-like C1-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIRPA is ubiquitously expressed. It is highly expressed in brain and detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. It is also detected on myeloid cells, but not T-cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. SIRPA is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. It mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: IHC AntibodiesImmune Checkpoint Detection: IP AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint TargetsImmunotherapyTargeted Therapy

参考文献

1. Timms JF. et al., 1999, Curr Biol. 9: 927-30. 2. Stofega MR. et al., 2000, J Biol Chem. 275: 28222-9. 3. Liu T. et al., 2005, J Proteome Res. 4: 2070-80. 4. Wolf-Yadlin A. et al., 2007, Proc Natl Acad Sci. 104: 5860-5.

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SIRP alpha Protein, Cynomolgus, Recombinant (His)

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