SIRP beta 1 isoform 3 Protein, Human, Recombinant (His & Avi)

SIRP beta 1 isoform 3 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 39.9 kDa and the accession number is Q5TFQ8-1.

SIRP beta 1 isoform 3 Protein, Human, Recombinant (His & Avi)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 112,000
500 μg	約5 days	￥ 447,500
1 mg	約5 days	￥ 746,000

バルクのお問い合わせ

説明	SIRP beta 1 isoform 3 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 39.9 kDa and the accession number is Q5TFQ8-1.

Species	Human
Expression Host	HEK293 Cells
Tag	C-His-Avi
Accession Number	Q5TFQ8-1
別名	SIRP-beta-1 isoform 3, SIRP-beta, SIRP-β-1 isoform 3, SIRP-β, FLJ26614, SIRPB1, CD172b, DKFZp686A05192, SIRP β1 isoform 3, 9930027N05Rik, Sirpb1a, Sirpb
Construction	Glu30-Leu371
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	39.9 kDa (predicted). Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Signal regulatory protein β1 (SIRPB1) is a signal regulatory protein member of the immunoglobulin superfamily and is capable of modulating receptor tyrosine kinase-coupled signaling. Copy number variations at the SIRPB1 locus were previously reported to associate with prostate cancer aggressiveness in patients.SIRPB1 gene amplification and overexpression were detected in prostate cancer specimens. The knockdown of SIRPB1 significantly suppressed cell growth in colony formation assays and cell mobility.

参考文献

1. Song Q, et al. SIRPB1 promotes prostate cancer cell proliferation via Akt activation. Prostate. 2020 Mar;80(4):352-364. doi: 10.1002/pros.23950. Epub 2020 Jan 6. PMID: 31905248; PMCID: PMC7421598.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

SIRP beta 1 isoform 3 Protein, Human, Recombinant (His & Avi)

参考文献

Calculator

技術サポート

Keywords