SMAD5 Protein, Mouse, Recombinant

SMAD5 Protein, Mouse, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 52.2 kDa and the accession number is P97454.

SMAD5 Protein, Mouse, Recombinant

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 142,000

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説明	SMAD5 Protein, Mouse, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 52.2 kDa and the accession number is P97454.

Species	Mouse
Expression Host	Baculovirus Insect Cells
Tag	Tag Free
Accession Number	P97454
別名	MusMLP, AI451355, Madh5, Dwf-C, 1110051M15Rik, SMAD family member 5
Construction	The Mouse SMAD5 (P97454) (Thr2-Ser465) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	52.2 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM Nacl, 10% glycerol, pH8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	SMAD5 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD5 is involved in the TGF-beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. It is also involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). The binding of ligands causes the oligomerization and phosphorylation of the SMAD5 protein. SMAD5 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.

参考文献

1. Vinayagam A. et al., 2011, Sci Signal. 4 (189): rs8. 2. Sangadala S. et al., 2007, J Biomol Struct Dyn. 25 (1): 11-23. 3. Riggins GJ. et al., 1996, Nat Genet. 13 (3): 347-9.

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SMAD5 Protein, Mouse, Recombinant

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