SNAP-25 Protein, Human, Recombinant (His)

カタログ番号 TMPY-02245

別名: synaptosomal-associated protein, 25kDa, SEC9, SNAP, RIC-4, dJ1068F16.2, SNAP-25, bA416N4.2, RIC4

Synaptosomal-associated protein 25, also known as Super protein, Synaptosomal-associated 25 kDa protein, SNAP25 and SNAP, is a cytoplasm and cell membrane protein that belongs to the SNAP-25 family. SNAP25 / SUP contains 2 t-SNARE coiled-coil homology domains. SNAP25 / SUP is a membrane bound protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP25 / SUP protein is a component of the SNARE complex, which is proposed to account for the specificity of membrane fusion and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. SNAP25 / SUP is a Q-SNARE protein contributing two α-helices in the formation of the exocytotic fusion complex in neurons where it assembles with syntaxin-1 and synaptobrevin. SNAP25 / SUP is involved in the molecular regulation of neurotransmitter release. It may play an important role in the synaptic function of specific neuronal systems. SNAP25 / SUP associates with proteins involved in vesicle docking and membrane fusion. SNAP25 / SUP regulates plasma membrane recycling through its interaction with CENPF. SNAP25 / SUP inhibits P/Q- and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca2+-independent fashion. In glutamatergic synapses SNAP25 / SUP decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.

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SNAP-25 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 73,500
200 μg	約5 days	￥ 125,500
500 μg	約5 days	￥ 255,000

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参考文献

生物学的情報

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説明

Species	Human
Expression Host	E. coli
Tag	His
Accession Number	P60880-1
別名	synaptosomal-associated protein, 25kDa, SEC9, SNAP, RIC-4, dJ1068F16.2, SNAP-25, bA416N4.2, RIC4
Construction	The Human SNAP25 (P60880-1) (Met 1-Gly 206) was expressed, with a polyhistide tag at the N-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	24.8 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Synaptosomal-associated protein 25, also known as Super protein, Synaptosomal-associated 25 kDa protein, SNAP25 and SNAP, is a cytoplasm and cell membrane protein that belongs to the SNAP-25 family. SNAP25 / SUP contains 2 t-SNARE coiled-coil homology domains. SNAP25 / SUP is a membrane bound protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP25 / SUP protein is a component of the SNARE complex, which is proposed to account for the specificity of membrane fusion and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. SNAP25 / SUP is a Q-SNARE protein contributing two α-helices in the formation of the exocytotic fusion complex in neurons where it assembles with syntaxin-1 and synaptobrevin. SNAP25 / SUP is involved in the molecular regulation of neurotransmitter release. It may play an important role in the synaptic function of specific neuronal systems. SNAP25 / SUP associates with proteins involved in vesicle docking and membrane fusion. SNAP25 / SUP regulates plasma membrane recycling through its interaction with CENPF. SNAP25 / SUP inhibits P/Q- and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca2+-independent fashion. In glutamatergic synapses SNAP25 / SUP decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.

参考文献

1. Hodel A , et al.,1998, Int. J. Biochem. Cell Biol. 30 (10): 1069-73. 2. Sudhof TC, et al.,2002, Nat Rev Neurosci 3 (8): 641-653. 3. Chapman ER et al.,2002, Nat. Rev. Mol. Cell Biol. 3(7): 498-508. 4. Chen X., et al., 2002, Neuron 33:397-409. 5. Huang Q., et al., 2008, FEBS Lett. 582:1431-1436.

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Keywords

SNAP-25 Protein, Human, Recombinant (His) synaptosomal-associated protein, 25kDa SEC9 SNAP RIC 4 SEC 9 RIC-4 SEC-9 SNAP 25 dJ1068F16.2 SNAP-25 bA416N4.2 SNAP25 RIC4 recombinant recombinant-proteins proteins protein