SP17 Protein, Mouse, Recombinant (His)

Sperm protein 17 (Sp17) is a highly conserved mammalian protein characterized in rabbit, mouse, monkey, baboon, macaque, human testis and spermatozoa. mRNA encoding Sp17 has been detected in a range of murine and human somatic tissues. It was also recognized in two myeloma cell lines and in neoplastic cells from patients with multiple myeloma and ovarian carcinoma. SP17 Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with N-His tag. The predicted molecular weight is 18.39 kDa and the accession number is Q62252.

SP17 Protein, Mouse, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 112,000
500 μg	約5 days	￥ 447,500
1 mg	約5 days	￥ 746,000

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説明

Species	Mouse
Expression Host	E. coli
Tag	N-His
Accession Number	Q62252
別名	SPA17, Sperm protein 17, CT22, Sp17-1
Construction	Met1-Asn149
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 90% as determined by HPLC
分子量	The protein has a predicted MW of 18.39 kDa. The protein migrates to 20-25 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Sperm protein 17 (Sp17) is a highly conserved mammalian protein characterized in rabbit, mouse, monkey, baboon, macaque, human testis and spermatozoa. mRNA encoding Sp17 has been detected in a range of murine and human somatic tissues. It was also recognized in two myeloma cell lines and in neoplastic cells from patients with multiple myeloma and ovarian carcinoma.

参考文献

1. Chiriva-Internati M, et al. Sperm protein 17 is expressed in the sperm fibrous sheath. J Transl Med. 2009 Jul 15;7:6doi: 10.1186/1479-5876-7-6PMID: 19604394; PMCID: PMC2727497.

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SP17 Protein, Mouse, Recombinant (His)

参考文献

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