Sphingosine Kinase 1/SPHK1 Protein, Human, Recombinant

Sphingosine Kinase 1/SPHK1 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 42.7 kDa and the accession number is Q9NYA1-1.

Sphingosine Kinase 1/SPHK1 Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 114,500

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説明	Sphingosine Kinase 1/SPHK1 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 42.7 kDa and the accession number is Q9NYA1-1.

Species	Human
Expression Host	Baculovirus-Insect Cells
Tag	Tag Free
Accession Number	Q9NYA1-1
別名	SPHK, SPHK1, sphingosine kinase 1, SPK
Construction	A DNA sequence encoding the human SPHK1 (Q9NYA1-1)(Met1-Leu384) was fused with two additional amino acids (Gly & Pro) at the N-terminus.
Protein Purity	> 85 % as determined by SDS-PAGE
分子量	42.7 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Formulation	Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Research Background	SPHK1, also known as sphingosine Kinase 1, catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P). S1P exhibits a broad spectrum of biological activities including cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis. It is a ligand for cell surface G protein-coupled receptors. SPHK 1 is a potential therapeutic target for the control of cancer and inflammation. SPHK1 plays a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes.

参考文献

1. Kohama T. et al., 1998, J Biol Chem. 273 (37): 23722-8. 2. Xia P. et al., 2002, J Biol Chem. 277 (10): 7996-8003. 3. Tsukahara T. et al., 2002, Tanpakushitsu Kakusan Koso. 47 (4): 509-13.

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Sphingosine Kinase 1/SPHK1 Protein, Human, Recombinant

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