SARS-COV-2 Spike S1 Protein (D614G, His & Avi)

The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct.

SARS-COV-2 Spike S1 Protein (D614G, His & Avi)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 117,500
500 μg	約5 days	￥ 468,500
1 mg	約5 days	￥ 782,500

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説明

Species	SARS-CoV-2
Expression Host	HEK293 Cells
Tag	C-His-Avi
Accession Number	A0A6G7K2L4
別名	Spike,S1 protein, Spike protein S1, S glycoprotein Subunit1, S1 protein
Construction	Gln14-Arg683(D614G)
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	77.9 kDa (predicted). Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct.

参考文献

1. Zhang L, et al. The D614G mutation in the SARS-CoV-2 spike protein reduces S1 shedding and increases infectivity. bioRxiv [Preprint]. 2020 Jun 12:2020.06.12.148726. doi: 10.1101/2020.06.12.148726. Update in: Nat Commun. 2020 Nov 26;11(1):6013. PMID: 32587973; PMCID: PMC731063

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SARS-COV-2 Spike S1 Protein (D614G, His & Avi)

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