SARS-COV-2 Spike S1 NTD Protein (His & Flag)

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. SARS-COV-2 Spike S1 NTD Protein (His & Flag) is expressed in HEK293 mammalian cells with C-His-Flag tag. The predicted molecular weight is 35 kDa and the accession number is A0A6G7K2L4.

SARS-COV-2 Spike S1 NTD Protein (His & Flag)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 117,500
500 μg	約5 days	￥ 468,500
1 mg	約5 days	￥ 782,500

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説明

Species	SARS-CoV-2
Expression Host	HEK293 Cells
Tag	C-His-Flag
Accession Number	A0A6G7K2L4
別名	S1 protein NTD,Spike glycoprotein Subunit1 NTD,S glycoprotein Subunit1 NTD,Spike protein S1 NTD
Construction	Val16-Ser305
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	35 kDa (predicted). Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

参考文献

1. Belouzard S, et al. Activation Of The Sars Coronavirus Spike Protein Via Sequential Proteolytic Cleavage At Two Distinct Sites[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(14):5871-5876.

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SARS-COV-2 Spike S1 NTD Protein (His & Flag)

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