Osteopontin Protein, Human, Recombinant (aa 17-300, His)

Osteopontin Protein, Human, Recombinant (aa 17-300, His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 34.8 kDa and the accession number is A0A024RDE6.

Osteopontin Protein, Human, Recombinant (aa 17-300, His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 107,000
500 μg	約5 days	￥ 424,500
1 mg	約5 days	￥ 711,500

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説明	Osteopontin Protein, Human, Recombinant (aa 17-300, His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 34.8 kDa and the accession number is A0A024RDE6.

Species	Human
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	A0A024RDE6
別名	BSP-1, Nephropontin, BNSP, ETA-1, OPN, Bone sialoprotein 1, BSPI, Uropontin, SPP-1, Osteopontin, SPP1
Construction	Ile17-Asn300
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	34.8 kDa (predicted). Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Ovarian cancer is one of the most lethal malignant tumors in women. Secreted phosphoprotein 1 (SPP1) plays an important role in some cancer types. The expression of SPP1 was higher in epithelial ovarian cancer tissues than in normal ovarian tissues. Silencing SPP1 decreased the cell proliferation, migration, and invasion. Ectopic expression of SPP1 increased the cell proliferation, migration, and invasion. Silencing SPP1 prevented ovarian cancer growth in mice. Silencing SPP1 inhibited Integrin β1/FAK/AKT pathway.

参考文献

1. Zeng B, et al. SPP1 promotes ovarian cancer progression via Integrin β1/FAK/AKT signaling pathway. Onco Targets Ther. 2018 Mar 12;11:1333-1343. doi: 10.2147/OTT.S154215. PMID: 29559792; PMCID: PMC5856063.

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Osteopontin Protein, Human, Recombinant (aa 17-300, His)

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