ST6GAL1 Protein, Mouse, Recombinant (His)

ST6GAL1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.9 kDa and the accession number is Q64685.

ST6GAL1 Protein, Mouse, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 103,500
200 μg	約5 days	￥ 177,000
500 μg	約5 days	￥ 358,000

バルクのお問い合わせ

生物学的情報	Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to N-Acetyllactosamine. The specific activity is > 150 pmol/min/μg.
説明	ST6GAL1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.9 kDa and the accession number is Q64685.

Species	Mouse
Expression Host	HEK293 Cells
Tag	His
Accession Number	Q64685
別名	ST6 β-galactosamide α-2,6-sialyltranferase 1, St6galI, ST6 beta-galactosamide alpha-2,6-sialyltranferase 1, Siat1, St6Gal-I, AW742324, ST6 beta-galactosamide α-2,6-sialyltranferase 1, St6gal
Construction	The extracellular domain of Mouse ST6GAL1 (NP_666045.1) (Lys 27-Cys 403) was fused with a polyhistidine tag at the N-terminus.
Protein Purity	> 96 % as determined by SDS-PAGE
分子量	45.9 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 120 mM NaCl, pH7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Beta-galactoside alpha-2,6-sialyltransferase 1, also known as B-cell antigen CD75, Sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, ST6GAL1 and SIAT1, is a single-pass type II membrane protein that belongs to the glycosyltransferase 29 family. Sialyltransferases are key enzymes in the biosynthesis of sialoglycoconjugates that catalyze the transfer of sialic residue from its activated form to an oligosaccharidic acceptor. ST6GAL1 / SIAT1 is normally found in the Golgi but can be proteolytically processed to a soluble form. It is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. β-Galactoside α2,6-sialyltransferases ST6GAL1 and ST6GAL2 are the two unique members of the ST6GAL family described in higher vertebrates. ST6GAL1 / SIAT1 transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.

参考文献

1. Collins,B.E. et al., 2006, Nat Immunol. 7(2):199-206. 2. Videira,P.A. et al., 2008, Glycoconj J. 25(3): 259-68. 3. Petit,D. et al., 2010, J Biol Chem. 285(49): 38399-414. 4. Kroes,R.A. et al., 2010, Proc Natl Acad Sci USA.107(28):12646-51.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

ST6GAL1 Protein, Mouse, Recombinant (His)

参考文献

Calculator

技術サポート

Keywords