TCN2 Protein, Human, Recombinant (His)

TCN2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 46.7 kDa and the accession number is P20062-1.

TCN2 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 138,000
100 μg	約5 days	￥ 236,500
200 μg	約5 days	￥ 402,000
500 μg	約5 days	￥ 812,500

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生物学的情報	Immobilized CD320 Protein, Mouse, Recombinant (hFc Tag) at 2 μg/mL (100 μL/well) can bind TCN2 Protein, Human, Recombinant (His Tag), the EC50 is 10-62 ng/mL.
説明	TCN2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 46.7 kDa and the accession number is P20062-1.

Species	Human
Expression Host	HEK293 Cells
Tag	His
Accession Number	P20062-1
別名	II, TC-2, D22S750, TC, TCII, TC2, transcobalamin II, D22S676
Construction	Human TCN2 (NP_000346.2) (Met 1-Trp 427) with a C-terminal polyhistidine tag
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	46.7 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Transcobalamin II, also known as TCN2 and TC II, is a plasma protein that binds cobalamin (Cbl; vitamin B12) as it is absorbed in the terminal ileum and distributes to tissues. The circulating transcobalamin II-cobalamin complex binds to receptors on the plasma membrane of tissue cells and is then internalized by receptor-mediated endocytosis. Transcobalamin II is a non-glycolated secretory protein of molecular mass 43 kDa. Its plasma membrane receptor (TC II-R) is a heavily glycosylated protein with a monomeric molecular mass of 62 kDa. Human TCN2 gene is composed of nine exons and eight introns spanning approximately 2 kb with multiple potential transcription start sites. A number of genetic abnormalities are characterized either by a failure to express TCN2 or by synthesis of an abnormal protein. The TCN2 deficiency results in cellular cobalamin deficiency, an early onset of megaloblastic anaemia, and neurological abnormalities.

参考文献

1. Rothenberg, S. P. et al., 1995, Baillieres Clin Haematol. 8 (3):499-514. 2. Bibi, H. et al., 1999, J Inherit Metab Dis. 22 (7):765-772. 3. Seetharam,B. et al.,2000, Vitam Horm. 59 :337-366.

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TCN2 Protein, Human, Recombinant (His)

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