Terminal deoxynucleotidyl transferase (TdT) is a highly conserved vertebrate enzyme that possesses the unique ability to catalyze the random addition of deoxynucleoside 5'-triphosphates onto the 3'-hydroxyl group of a single-stranded DNA. It plays an important role in the generation of immunoglobin and T-cell receptor diversity. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
10 μg | 約5 days | ¥ 35,500 |
50 μg | 約5 days | ¥ 107,000 |
500 μg | 約5 days | ¥ 535,000 |
1 mg | 約5 days | ¥ 801,000 |
説明 | Terminal deoxynucleotidyl transferase (TdT) is a highly conserved vertebrate enzyme that possesses the unique ability to catalyze the random addition of deoxynucleoside 5'-triphosphates onto the 3'-hydroxyl group of a single-stranded DNA. It plays an important role in the generation of immunoglobin and T-cell receptor diversity. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. |
Species | Human |
Expression Host | E. coli |
Tag | N-6xHis |
Accession Number | P04053 |
別名 | Terminal transferase, Terminal deoxynucleotidyltr, DNTT, Terminal deoxynucleotidyltransferase, DNA nucleotidylexotransferase, TDT, Terminal addition enzyme |
Amino Acid | Met1-Ala509 |
Construction | Met1-Ala509 |
Protein Purity | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
分子量 | 62 KDa (reducing condition) |
Endotoxin | < 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
Formulation | Supplied as a 0.2 μm filtered solution of 0.1M KH<sub>2</sub>PO<sub>4</sub>, 200 mM KCl, 1 mM 2-Mercaptoethanol, 50% Glycerol, pH 7.2. |
Stability & Storage |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
Research Background | Terminal deoxynucleotidyl transferase (TdT) is a highly conserved vertebrate enzyme that possesses the unique ability to catalyze the random addition of deoxynucleoside 5'-triphosphates onto the 3'-hydroxyl group of a single-stranded DNA. It plays an important role in the generation of immunoglobin and T-cell receptor diversity. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. |
bottom
Please read the User Guide of Recombinant Proteins for more specific information.
TDT Protein, Human, Recombinant (His) Terminal transferase Terminal deoxynucleotidyltr DNTT Terminal deoxynucleotidyltransferase DNA nucleotidylexotransferase TDT Terminal addition enzyme recombinant recombinant-proteins proteins protein