TGFBR2 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 17.8 kDa and the accession number is P37173.
パッケージサイズ | 在庫状況 | 単価(税別) |
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100 μg | 在庫あり | ¥ 73,500 |
200 μg | 約5 days | ¥ 141,500 |
500 μg | 約5 days | ¥ 344,000 |
生物学的情報 |
Measured by its binding ability in a functional ELISA. Immobilized TGFBR2h (1-166Q) at 10 μg/mL (100 μL/well) can bind TGFB1-His/Biotin , the EC50 of human TGFB1-His/Biotin is 130-300 ng/mL.
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説明 | TGFBR2 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 17.8 kDa and the accession number is P37173. |
Species | Human |
Expression Host | Baculovirus Insect Cells |
Tag | C-His |
Accession Number | P37173 |
別名 | LDS2B, FAA3, TAAD2, MFS2, TGFR-2, TGFbeta-RII, transforming growth factor, beta receptor II (70/80kDa), LDS2, transforming growth factor, β receptor II, LDS1B, AAT3, TGFβ-RII, RIIC |
Construction | The Human TGFBR2 (NP_003233.4) (Met1-Gln166) was expressed with a polyhistidine tag at the C-terminus. |
Protein Purity |
> 90 % as determined by SDS-PAGE.
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分子量 | 17.8 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PBS, 150 mM NaCl, 10% glycerol, 0.5 mM TCEP, pH 7.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | TGFBR2 is a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. It is a transmembrane protein. TGFBR2 is comprised of a C-terminal protein kinase domain and an N-terminal ectodomain. The ectodomain consists of a compact fold containing nine beta-strands and a single helix stabilized by a network of six intra strand disulfide bonds. The folding topology includes a central five-stranded antiparallel beta-sheet, eight-residues long at its centre, covered by a second layer consisting of two segments of two-stranded antiparallel beta-sheets. TGFBR2 has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in TGFBR2 gene have been associated with Marfan syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. TGFBR2 attenuates the biological activities of TGF-beta in colorectal cancer. TGFBR2 expression is increased in oral squamous cell carcinoma cells. Its expression is decreased by IL-1beta while inducing Sp3 via NFkappaB. TGFB2 and TGFBR2 are involved in the antiestrogenic activity. |
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Please read the User Guide of Recombinant Proteins for more specific information.
TGFBR2 Protein, Human, Recombinant (His) AAT-3 FAA-3 LDS2B AAT 3 TGFR2 FAA 3 FAA3 TAAD2 MFS2 TGFR-2 transforming growth factor, beta receptor II TGFbeta-RII transforming growth factor, beta receptor II (70/80kDa) MFS-2 LDS2 MFS 2 transforming growth factor, β receptor II LDS-2 TGFb-RII LDS1B TAAD 2 AAT3 LDS 2 transforming growth factor, b receptor II TGFβ-RII 70/80kDa TGFR 2 TAAD-2 RIIC recombinant recombinant-proteins proteins protein