TGFBR2 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 25-38 KDa and the accession number is Q62312-2.
| 生物学的情報 |
Measured by its ability to inhibit TGF-beta 1 activity on TF‑1 human erythroleukemic cells. The ED50 for this effect is 1680.39ng/ml in the presence of 1ng/ml of recombinant human TGF-beta 1. (Regularly tested)
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| 説明 |
TGFBR2 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 25-38 KDa and the accession number is Q62312-2. |
| Species |
Mouse
|
| Expression Host |
Human Cells
|
| Tag |
C-6His
|
| Accession Number |
Q62312-2
|
| 別名 |
TGFR-2, TGF-beta type II receptor, Transforming growth factor-beta receptor type II, TGF-β type II receptor, TβR-II, TbetaR-II, TGF-β receptor type II, Transforming growth factor-β receptor type II, TGF-β receptor type-2, Tgfbr2, TGF-beta receptor type-2, TGF-beta receptor type II
|
| Amino Acid |
Ile24-Asp159
|
| Construction |
Recombinant Mouse Transforming Growth Factor-beta Receptor Type II is produced by our Mammalian expression system and the target gene encoding Ile24-Asp159 is expressed with a 6His tag at the C-terminus.
|
| Protein Purity |
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
|
| 分子量 |
25-38 KDa, reducing conditions
|
| Endotoxin |
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
|
| Formulation |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
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| Reconstitution |
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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| Stability & Storage |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
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| Shipping |
The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below.
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| Research Background |
Transforming growth factor-β (TGF-β) is an essential regulator in the processes of development, cell proliferation, and extracellular matrix deposition. TGF-β regulates cellular processes by binding to three high-affinity cell surface receptors: TGF-β receptor type I (TGF-β-RI), TGF-β receptor type II (TGF-β-RII), and TGF-ββ receptor type III (TGF-β-RIII). TGF-β RII is consists of a C-terminal protein kinase domain and an N-terminal ectodomain and belongs to transforming growth factor-beta (TGF-β) receptor subfamily. TGF-β RII has a protein kinase domain which can form a heterodimeric complex with another receptor protein and bind TGF-beta. This receptor/ligand complex phosphorylates protein will enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation.
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