THRB Protein, Human, Recombinant (His)

カタログ番号 TMPY-06821

別名: THRB1, GRTH, THR1, ERBA2, C-ERBA-2, THRB2, thyroid hormone receptor, β, PRTH, C-ERBA-β, thyroid hormone receptor, beta, C-ERBA-BETA, NR1A2

Thyroid hormone receptor beta (THRB), also known as TRbeta and NR1A2, belongs to the nuclear hormone receptor family. Featuring an N-terminal transactivation domain, a central DNA-binding domain, and a hormone/ligand-binding domain, THRB isoform 1/2 expression localizes predominately to the liver and pituitary, respectively. THRB regulates the feedback of T3-dependent thyrotropin-stimulating hormone (TSH) transcription in the pituitary by binding to thyroid hormone (TH) response elements present on promoters of TH-responsive genes. Located on chromosome 3p24, THRB gene mutation has been linked to autosomal dominant resistance to TH (RTH), which is characterized by goiter and elevated levels of T3, T4, and TSH. THRB is a known pharmacological target for the treatment of non-alcoholic steatohepatitis (NASH). THRB may be a target for future cancer therapies since its knockdown enhanced the viability of endometrial cancer cells and activated the mTOR-4EBP1/eIF4G pathway.

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THRB Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 138,000

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生物学的特性に関する説明

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Product Properties

参考文献

説明

Species	Human
Expression Host	E. coli
Tag	His
Accession Number	P10828-1
別名	THRB1, GRTH, THR1, ERBA2, C-ERBA-2, THRB2, thyroid hormone receptor, β, PRTH, C-ERBA-β, thyroid hormone receptor, beta, C-ERBA-BETA, NR1A2
Construction	The Human THRB (P10828-1) (Glu202-Asp461) was expressed, with a polyhistidine tag at the N-terminus.
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	30.33 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 1mm Tcep, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Thyroid hormone receptor beta (THRB), also known as TRbeta and NR1A2, belongs to the nuclear hormone receptor family. Featuring an N-terminal transactivation domain, a central DNA-binding domain, and a hormone/ligand-binding domain, THRB isoform 1/2 expression localizes predominately to the liver and pituitary, respectively. THRB regulates the feedback of T3-dependent thyrotropin-stimulating hormone (TSH) transcription in the pituitary by binding to thyroid hormone (TH) response elements present on promoters of TH-responsive genes. Located on chromosome 3p24, THRB gene mutation has been linked to autosomal dominant resistance to TH (RTH), which is characterized by goiter and elevated levels of T3, T4, and TSH. THRB is a known pharmacological target for the treatment of non-alcoholic steatohepatitis (NASH). THRB may be a target for future cancer therapies since its knockdown enhanced the viability of endometrial cancer cells and activated the mTOR-4EBP1/eIF4G pathway.

参考文献

1. T. Parry, D. Ledee, M.S. Willis, M.A. Portman, Chapter 10 - Nuclear Receptors and the Adaptive Response of the Heart, Editor(s): Jonathan C. Schisler, Charles H. Lang, Monte S. Willis, Endocrinology of the Heart in Health and Disease, Academic Press, 2017. 2. Lai HP, Chen MH. Atrial Fibrillation with Heart Failure in a Case with Resistance to Thyroid Hormone Due to a Rare Thyroid Hormone Receptor β Gene Mutation. Int J Mol Sci. 2022. 3. Ke S, et al. Thyroid hormone receptor β sumoylation is required for thyrotropin regulation and thyroid hormone production. JCI Insight. 2021. 4. Krystian Jazdzewski, et al. Thyroid Hormone Receptor β (THRB) Is a Major Target Gene for MicroRNAs Deregulated in Papillary Thyroid Carcinoma (PTC), The Journal of Clinical Endocrinology & Metabolism, 2011. 5. Ren B, et al. Thyroid Hormone Receptor β Knockdown Reduces Suppression of Progestins by Activating the mTOR Pathway in Endometrial Cancer Cells. International Journal of Molecular Sciences. 2022.

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Keywords

THRB Protein, Human, Recombinant (His) THRB1 THR-1 THRB-1 GRTH THR1 ERBA2 C-ERBA-2 THRB 2 THRB 1 THRB2 THRB-2 THR 1 thyroid hormone receptor, β ERBA 2 PRTH C-ERBA-β thyroid hormone receptor, beta C-ERBA-BETA NR1A2 ERBA-2 recombinant recombinant-proteins proteins protein