Thyroid Peroxidase Protein, Mouse, Recombinant (His)

Thyroid peroxidase, also called thyroperoxidase (TPO) or iodide peroxidase that encodes a 933 amino-acid residue (aa) molecule with a single membrane-spanning region. Thyroid peroxidase plays a key role in thyroid hormone synthesis by catalyzing both the iodination of tyrosine residues to form monoiodotyrosine (MIT) and diiodotyrosine (DIT) residues and the coupling of iodotyrosine residues in Tg, resulting in the formation of T3 and T4. It is a frequent epitope of autoantibodies in autoimmune thyroid disease, for example, the expression of thyroid peroxidase is lost in papillary thyroid carcinoma.

Thyroid Peroxidase Protein, Mouse, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 107,000
500 μg	約5 days	￥ 424,500
1 mg	約5 days	￥ 711,500

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説明

Species	Mouse
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	P35419
別名	MK-CSF, MGDF, ML, MPLLG, THPO, TPOMKCSF, Thrombopoietin, MKCSF, MGDFC-mpl ligand, THCYT1, TPO, MGC163194, MPL ligand
Construction	Gly32-Arg834
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	90.6 kDa (predicted). Due to glycosylation, the protein migrates to 100-115 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Thyroid peroxidase, also called thyroperoxidase (TPO) or iodide peroxidase that encodes a 933 amino-acid residue (aa) molecule with a single membrane-spanning region. Thyroid peroxidase plays a key role in thyroid hormone synthesis by catalyzing both the iodination of tyrosine residues to form monoiodotyrosine (MIT) and diiodotyrosine (DIT) residues and the coupling of iodotyrosine residues in Tg, resulting in the formation of T3 and T4. It is a frequent epitope of autoantibodies in autoimmune thyroid disease, for example, the expression of thyroid peroxidase is lost in papillary thyroid carcinoma.

参考文献

1. Ruf J, Carayon P. Structural and functional aspects of thyroid peroxidase. Arch Biochem Biophys. 2006 Jan 15;445(2):269-77. doi: 10.1016/j.abb.2005.06.023. Epub 2005 Jul 27. PMID: 16098474. 2. Chardès T, et al. The human anti-thyroid peroxidase autoantibody repertoire in Graves' and Hashimoto's autoimmune thyroid diseases. Immunogenetics. 2002 Jun;54(3):141-57. doi: 10.1007/s00251-002-0453-9. Epub 2002 May 3. PMID: 12073143 3. Shield PW, et al. Identification of metastatic papillary thyroid carcinoma in FNA specimens using thyroid peroxidase immunohistochemistry. Cytopathology. 2018 Jun;29(3):227-232. doi: 10.1111/cyt.1253Epub 2018 Mar 6. PMID: 29508480.

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Thyroid Peroxidase Protein, Mouse, Recombinant (His)

参考文献

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