TIM-3/KIM-3/HAVCR2 Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 21.3 kDa and the accession number is AAL65157.1.
パッケージサイズ | 在庫状況 | 単価(税別) |
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20 μg | 在庫あり | ¥ 45,500 |
100 μg | 約5 days | ¥ 107,500 |
200 μg | 約5 days | ¥ 183,000 |
500 μg | 約5 days | ¥ 576,000 |
生物学的情報 |
Labeled biotin to TIM-3 Protein, Human, Recombinant (His Tag) by a certain molar ratio;Using the Octet RED System, the affinity constant (Kd) of TIM-3 Protein, Human, Recombinant (His Tag), Biotinylated bound to Anti-TIMD3 Antibody was 0.1 nM.
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説明 | TIM-3/KIM-3/HAVCR2 Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 21.3 kDa and the accession number is AAL65157.1. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | C-His |
Accession Number | AAL65157.1 |
別名 | TIMD3, hepatitis A virus cellular receptor 2, CD366, KIM-3, TIMD-3, Tim-3, HAVcr-2, HAVCR2, TIM3 |
Construction | The Human HAVCR2 (AAL65157.1) (Met1-Arg200) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro. |
Protein Purity |
> 80 % as determined by SDS-PAGE.
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分子量 | 21.3 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Hepatitis A virus cellular receptor 2 (HAVCR2), formerly known as T cell immunoglobulin and mucin domain-3 (TIM-3), is a transmembrane glycoprotein expressed on the surface of terminally differentiated Th1 cells but not on Th2 cells. It was the first surface molecule that specifically identifies Th1 cells in both the mouse and human. Recently, the identification of Galectin-9 as a ligand for TIM-3 has established the TIM-3-Galectin-9 pathway as an important regulator of Th1 immunity and tolerance induction. Engagement of Tim-3 by its ligand galectin-9 negatively regulates IFN-gamma secretion and influences the ability to induce T cell tolerance in both mice and man. It suggests a novel paradigm in which dysregulation of the TIM-3-galectin-9 pathway could underlie chronic autoimmune disease states, such as multiple sclerosis. Recent work has explored the role of TIM-3 in systemic lupus erythematosus (SLE), and their results indicate that TIM-3 may represent a novel target for the treatment of SLE. Numerous studies have demonstrated that Tim-3 influences autoimmune diseases, including diabetes and multiple sclerosis, and its role in other inflammatory diseases including allergies and cancer is beginning to become clear. In the tumor rejection model, the soluble form of Tim-3 (sTim-3) significantly impaired T cell antitumor immunity, evidenced by decreased antitumor CTL activity and reduced amount of tumor-infiltrating lymphocytes in the tumor. sTim-3 as an immunoregulatory molecule that may be involved in the negative regulation of T cell-mediated immune response.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: IP AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy |
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Please read the User Guide of Recombinant Proteins for more specific information.
TIM-3/KIM-3/HAVCR2 Protein, Human, Recombinant (His), Biotinylated TIMD3 hepatitis A virus cellular receptor 2 CD 366 TIM 3 CD-366 CD366 TIMD 3 KIM-3 TIMD-3 Tim-3 HAVcr-2 HAVCR2 KIM 3 KIM3 TIM-3 HAVCR 2 TIM3 recombinant recombinant-proteins proteins protein