TIMP-2 Protein, Human, Recombinant

TIMP-2 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 22 kDa and the accession number is A0A140VK57.

TIMP-2 Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
20 μg	約5 days	￥ 82,000
100 μg	約5 days	￥ 207,500

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説明	TIMP-2 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 22 kDa and the accession number is A0A140VK57.

Species	Human
Expression Host	HEK293 Cells
Tag	Tag Free
Accession Number	A0A140VK57
別名	CSC-21K, DDC8, TIMP metallopeptidase inhibitor 2
Construction	The mature form of Human TIMP2 (NP_003246.1) (Cys 27-Pro 220) was expressed and purified.
Protein Purity	> 96 % as determined by SDS-PAGE
分子量	22 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Tissue inhibitors of metalloproteinases (TIMP) family are natural inhibitors of the matrix metalloproteinases (MMPs), the zinc enzymes involved in extracellular matrix maintenance and remodeling. The TIMP family encompasses four members (TIMP1-4), and they inhibit most MMPs by forming non-covalent binary complex. TIMP2 is a 22 kDa non N-glycosylated protein expressed by a variety of cell types, and plays a unique role among TIMP family members owing to its functions to regulate cellular responses to growth factors. Findings establish an unexpected, MMP-independent mechanism for TIMP2 inhibition of endothelial cell proliferation in vitro and reveal an important component of the antiangiogenic effect of TIMP2 in vivo. TIMP-2 thus is critical to the maintenance of tissue homeostasis and is involved in the regulation of tumor microenvironment.

参考文献

1. Stetler-Stevenson, W.G. et al., 1992, Matrix. Suppl.1: 299-306. 2. Stetler-Stevenson, W.G. et al., 2005, Trends. Mol. Med. 11: 97-103. 3. Seo, D.W. et al., 2003, Cell. 114: 171-180.

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TIMP-2 Protein, Human, Recombinant

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