Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. Tissue Inhibitor of Metalloproteinases 2 (TIMP-2) is a non N-glycosylated protein with a molecular mass of 22 kDa. It produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes and irreversibly inactivates them by binding to their catalytic zinc cofactor. TIMP-2 also has erythroid‑potentiating and cell growth promoting activities.
| 説明 |
Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. Tissue Inhibitor of Metalloproteinases 2 (TIMP-2) is a non N-glycosylated protein with a molecular mass of 22 kDa. It produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes and irreversibly inactivates them by binding to their catalytic zinc cofactor. TIMP-2 also has erythroid‑potentiating and cell growth promoting activities. |
| Species |
Human
|
| Expression Host |
Human Cells
|
| Tag |
C-6His
|
| Accession Number |
P16035
|
| 別名 |
Tissue Inhibitor of Metalloproteinases 2, TIMP2, CSC-21K, TIMP-2, Metalloproteinase Inhibitor 2
|
| Amino Acid |
Cys27-Pro220
|
| Construction |
Recombinant Human Tissue Inhibitor of Metalloproteinases 2 is produced by our Mammalian expression system and the target gene encoding Cys27-Pro220 is expressed with a 6His tag at the C-terminus.
|
| Protein Purity |
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
|
| 分子量 |
26 KDa, reducing conditions
|
| Endotoxin |
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
|
| Formulation |
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
|
| Reconstitution |
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
|
| Stability & Storage |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
|
| Shipping |
The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below.
|
| Research Background |
Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. Tissue Inhibitor of Metalloproteinases 2 (TIMP-2) is a non N-glycosylated protein with a molecular mass of 22 kDa. It produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes and irreversibly inactivates them by binding to their catalytic zinc cofactor. TIMP-2 also has erythroid‑potentiating and cell growth promoting activities.
|
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