TLK1 Protein, Human, Recombinant (His & GST)

Tousled-like kinase 1 (or protein kinase ubiquitous, PKU-beta/TLK1) is a serine/threonine protein kinase that is implicated in chromatin remodeling, DNA replication and mitosis. TLK1 has a function important for proper chromosome segregation and maintenance of diploid cells at mitosis in mammalian cells that could be mediated by reduced phosphorylation of histone H3 and condensation of chromosomes, although other explanations to the phenotype are possible.

TLK1 Protein, Human, Recombinant (His & GST)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 114,500

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説明

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	His, GST
Accession Number	Q9UKI8-1
別名	KIAA0137, PKU-beta, tousled like kinase 1, PKU-β
Construction	The Human TLK1 isoform 1 (Q9UKI8-1) (Met 1-Tyr 766) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Protein Purity	> 88 % as determined by SDS-PAGE
分子量	114 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10% gly.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Research Background	Tousled-like kinase 1 (or protein kinase ubiquitous, PKU-beta/TLK1) is a serine/threonine protein kinase that is implicated in chromatin remodeling, DNA replication and mitosis. TLK1 has a function important for proper chromosome segregation and maintenance of diploid cells at mitosis in mammalian cells that could be mediated by reduced phosphorylation of histone H3 and condensation of chromosomes, although other explanations to the phenotype are possible.

参考文献

1. Sillje H.H.W., et al., 2001, Curr. Biol. 11:1068-73. 2. Beausoleil S.A., et al., 2004, Proc. Natl. Acad. Sci. USA. 101:12130-5. 3. Oppermann F.S., et al., 2009, Mol. Cell. Proteomics 8:1751-64. 4. Mayya V., et al., 2009, Sci. Signal. 2: RA46-RA46.

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TLK1 Protein, Human, Recombinant (His & GST)

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