TLK2 Protein, Human, Recombinant (aa 397-772, His & GST)

TLK2 Protein, Human, Recombinant (aa 397-772, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 71.3 kDa and the accession number is Q86UE8-1.

TLK2 Protein, Human, Recombinant (aa 397-772, His & GST)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 114,500

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説明	TLK2 Protein, Human, Recombinant (aa 397-772, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 71.3 kDa and the accession number is Q86UE8-1.

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	His, GST
Accession Number	Q86UE8-1
別名	PKU-α, tousled-like kinase 2, HsHPK, PKU-ALPHA
Construction	The amino acids (Leu 397-Asn 772) of Human TLK2 (Q86UE8-1) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	71.3 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 20 mM Tris, 500 mM NaCl, 0.5 mM PMSF, pH 8.0.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Research Background	Serine / threonine-protein kinase tousled-like 2, also known as PKU-alpha, Tousled-like kinase 2 and TLK2, is a nucleus protein which belongs to the protein kinase superfamily and Ser/Thr protein kinase family. The tousled-like kinases are an evolutionarily conserved family of proteins implicated in DNA repair, DNA replication and mitosis in metazoans and plants. Their absence from the yeasts and other eukaryotic 'microbes' suggests a specific role for them in the development of multicellular organisms. Tousled-like kinase 2 / TLK2 is widely expressed. It is present in fetal placenta, liver, kidney, pancreas, heart and skeletal muscle. It is also found in adult cell lines. Tousled-like kinase 2 / TLK2 contains one protein kinase domain. Tousled-like kinase 2 / TLK2 is rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly.

参考文献

1. Sillje HHW. et al., 2001,Curr Biol. 11: 1068-73. 2. Groth A. et al., 2003, EMBO J. 22: 1676-87. 3. Beausoleil SA. et al., 2004, Proc Natl Acad Sci. 101: 12130-5. 4. Mayya V. et al., 2009, Sci Signal. 2: RA46. 5. Oppermann FS. et al., 2009, Mol Cell Proteomics. 8: 1751-64.

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TLK2 Protein, Human, Recombinant (aa 397-772, His & GST)

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