UCHL1 Protein, Human, Recombinant (His)

UCHL1 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 25.6 kDa and the accession number is P09936.

UCHL1 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 91,500
500 μg	約5 days	￥ 594,500

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説明	UCHL1 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 25.6 kDa and the accession number is P09936.

Species	Human
Expression Host	E. coli
Tag	His
Accession Number	P09936
別名	Uch-L1, PGP9.5, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase), HEL-117, PGP95, PARK5, NDGOA
Construction	The native Human UCHL1 (NP_004172.2) (Gln 2-Ala 223) was expressed, with a polyhistide tag at the N-terminus.
Protein Purity	> 92 % as determined by SDS-PAGE
分子量	25.6 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Supplied as sterile 20 mM Tris, 500 mM NaCl, 20% glycerol, 1 mM DTT, pH 8.0.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqutinating enzyme with important functions in recycling of ubiquitin. Regulated proteolysis by the ubiquitin pathway has been implicated in control of the cell cycle, transcriptional activation, cell fate and growth, and synaptogenesis. The ubiquitin-proteasome system is involved in synaptic plasticity and is proposed to be part of a molecular switch that converts short-term synaptic potentiation to long-term changes in synaptic strength. UCHL1 is found in neuronal cell bodies and processes throughout the neocortex (at protein level). It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. UCHL1 is weakly expressed in ovary. UCHL1 is a ubiquitin-protein hydrolase. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. UCHL1 dysfunction has been associated with neurodegeneration in Parkinson's, Alzheimer's, and Huntington's disease patients. Reduced UCHL1 function may jeopardize the survival of CNS neurons.

参考文献

1. Wada H., et al., 1998, Biochem. Biophys. Res. Commun. 251:688-92. 2. Choi J., et al., 2004, J. Biol. Chem. 279:13256-64. 3. Lombardino,A.2005, et al., J.Proc Natl Acad Sci.USA102 (22):8036-41 4. Okochi-Takada,E. et al., 2006, Int J Cancer. 119 (6):1338-44. 5. Zetterberg, M. et al., 2010, Mol Neurodegener 5 :11.

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UCHL1 Protein, Human, Recombinant (His)

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