UCHL3 Protein, Mouse, Recombinant (His)

UCHL3 Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 27.5 kDa and the accession number is Q9JKB1.

UCHL3 Protein, Mouse, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 138,000

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説明	UCHL3 Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 27.5 kDa and the accession number is Q9JKB1.

Species	Mouse
Expression Host	E. coli
Tag	N-His
Accession Number	Q9JKB1
別名	ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Construction	The Mouse UCHL3 (Q9JKB1) (Glu 2-Ala 230) was expressed, with a polyhistide tag at the N-terminus.
Protein Purity	> 97 % as determined by SDS-PAGE
分子量	27.5 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Supplied as sterile 50 mM Tris, 150 mM NaCl, 20% glycerol, pH 7.7.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase that belongs to the peptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotypes indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer.

参考文献

1. Wood,M.A. et al., 2005, Hippocampus 15 (5):610-21. 2. Kwon,J. et al., 2006, Exp Anim 55 (1):35-43. 3. Setsuie,R. et al., 2009, Neurochem Int 54 (5-6):314-21. 4. Setsuie,R. et al., 2010, Neurochem Int 56 (8):911-8.

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UCHL3 Protein, Mouse, Recombinant (His)

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