USP7 Protein, Human, Recombinant (aa 208-560, His & GST)

USP7 Protein, Human, Recombinant (aa 208-560, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 68.8 kDa and the accession number is Q93009-1.

USP7 Protein, Human, Recombinant (aa 208-560, His & GST)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 114,500

バルクのお問い合わせ

説明	USP7 Protein, Human, Recombinant (aa 208-560, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 68.8 kDa and the accession number is Q93009-1.

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	His, GST
Accession Number	Q93009-1
別名	ubiquitin specific peptidase 7 (herpes virus-associated), HAUSP, TEF1
Construction	The amino acid sequence (Lys 208-Glu 560) of Human USP7 (NP_003461.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	68.8 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 0.5 mM EDTA, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Ubiquitin carboxyl-terminal hydrolase 7, also known as Ubiquitin thioesterase 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin-specific-processing protease 7, USP7 and HAUSP, is a widely expressed protein that belongs to the peptidase C19 family. USP7 is a member of the family of deubiquitinating enzymes. It is involved in the regulation of stress response pathways, epigenetic silencing and the progress of infections by DNA viruses. USP7 is a protein with a cysteine peptidase core, N- and C-terminal domains required for protein-protein interactions. USP7 contributes to epigenetic silencing of homeotic genes by Polycomb (Pc). USP7 cleaves ubiquitin fusion protein substrates. It deubiquitinates TP53/p53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2. USP7 also induces TP53-dependent cell growth repression and apoptosis. USP7 has key roles in the p53 pathway whereby it stabilizes both p53 and MDM2. Herpes simplex virus type 1 (HSV-1) regulatory protein ICP stimulates lytic infection and the reactivation of quiescent viral genomes. ICP interacts very strongly with USP7. USP7-mediated stabilization of ICP is dominant over ICP-induced degradation of USP7 during productive HSV-1 infection. The biological significance of the ICP-USP7 interaction may be most pronounced in natural infection situations, in which limited amounts of ICP are expressed.

参考文献

1. Zakut-Houri R.,et al.,(1985), Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells. EMBO J. 4:1251-1255. 2. Lamb P.,et al., (1986), Characterization of the human p53 gene.Mol. Cell. Biol. 6:1379-1385. 3. Harlow E.,et al.,(1985), Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53.Mol. Cell. Biol. 5:1601-1610.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

USP7 Protein, Human, Recombinant (aa 208-560, His & GST)

参考文献

Calculator

技術サポート

Keywords