VEGF164 Protein, Mouse, Recombinant

VEGF164 Protein, Mouse, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 19.4 kDa and the accession number is Q00731-2.

VEGF164 Protein, Mouse, Recombinant

パッケージサイズ	在庫状況	単価(税別)
5 μg	在庫あり	￥ 17,500
10 μg	約5 days	￥ 28,500
20 μg	約5 days	￥ 45,500
50 μg	約5 days	￥ 85,500
100 μg	約5 days	￥ 137,000
200 μg	約5 days	￥ 222,500
500 μg	約5 days	￥ 652,000

バルクのお問い合わせ

生物学的情報	Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 5-22 ng/mL.
説明	VEGF164 Protein, Mouse, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 19.4 kDa and the accession number is Q00731-2.

Species	Mouse
Expression Host	Baculovirus-Insect Cells
Tag	Tag Free
Accession Number	Q00731-2
別名	vascular endothelial growth factor A, Vpf, Vegf
Construction	A DNA sequence encoding the mouse VEGF164 (isoform VEGF-1) (Q00731-2) (Met 1-Arg 190) was expressed and purified.
Protein Purity	≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
分子量	19.4 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile 100mM Glycine, 10mM NaCl, pH7.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

1. Woolard J.et al.(2004) VEGF165b, an inhibitory vascular endothelial growth factor splice variant: mechanism of action, in vivo effect on angiogenesis and endogenous protein expression. Cancer Res. 64(21): 7822-7835. 2. Jia SF,et al.(2008) VEGF165 is necessary to the metastatic potential of Fas(-) osteosarcoma cells but will not rescue the Fas(+) cells. J Exp Ther Oncol. 7(2): 89-97. 3. Cimpean AM,et al.(2008) Vascular endothelial growth factor A (VEGF A) as individual prognostic factor in invasive breast carcinoma. Rom J Morphol Embryol. 49(3): 303-8. 4. HAmdollah Zadeh MA,et al.(2008) VEGF-mediated elevated intracellular calcium and angiogenesis in human microvascular endothelial cells in vitro are inhibited by dominant negative TRPC6. Microcirculation. 15(7): 605-14. 5. Eisenach PA,et al.(2010) MT1-MMP regulates VEGF-A expression through a complex with VEGFR-2 and Src. J Cell Sci. 123(Pt 23):4182-4193.Claesson-Welsh L (2010) Gremlin: vexing VEGF receptor agonist. Blood. 116(18):3386-7.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

VEGF164 Protein, Mouse, Recombinant

参考文献

Calculator

技術サポート

Keywords