VEGFC Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 15.5 kDa and the accession number is P49767.
パッケージサイズ | 在庫状況 | 単価(税別) |
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5 μg | 在庫あり | ¥ 13,000 |
10 μg | 約5 days | ¥ 19,000 |
20 μg | 約5 days | ¥ 28,000 |
50 μg | 約5 days | ¥ 46,500 |
100 μg | 約5 days | ¥ 68,000 |
生物学的情報 |
1. Measured by its binding ability in a functional ELISA. Immobilized VEGF C-his at 2 μg/mL (100 μL/well) can bind VEGFR3 hFc, the EC50 of VEGFR3 hFc is 2-15 ng/mL.
2. Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4 nM.
3. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 0.1-0.5 μg/mL.
4. Captured VEGFR3/FLT4 Protein, Human on anti-Human IgG Fc via CM5 Chip can bind VEGF-C with an affinity constant of 31.82 pM as determined in a SPR assay (Routinely tested).
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説明 | VEGFC Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 15.5 kDa and the accession number is P49767. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | C-His |
Accession Number | P49767 |
別名 | LMPH1D, VEGF-C, Flt4-L, vascular endothelial growth factor C, VRP |
Construction | mature form of Human VEGFC (NP_005420.1) (Thr103-Arg227) |
Protein Purity |
> 95 % as determined by SDS-PAGE
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分子量 | 15.5 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Vascular endothelial growth factor C (VEGF-C) is a member of the VEGF family. Upon biosynthesis, VEGF-C protein is secreted as a non-covalent momodimer in an anti-parellel fashion. VEGF-C protein is a dimeric glycoprotein, as a ligand for two receptors, VEGFR-3 (Flt4), and VEGFR-2. VEGF-C may function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis. VEGF-C protein is over-expressed in various human cancers including breast cancer and prostate cancer. VEGF-C/VEGFR-3 axis, through different signaling pathways, plays a critical role in cancer progression by regulating different cellular functions, such as invasion, proliferation, and resistance to chemotherapy. Thus, targeting the VEGF-C/VEGFR-3 axis may be therapeutically significant for certain types of tumors. |
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Please read the User Guide of Recombinant Proteins for more specific information.
VEGFC Protein, Human, Recombinant (His) LMPH1D VEGF-C Flt4-L vascular endothelial growth factor C VRP recombinant recombinant-proteins proteins protein