Von Willebrand Factor/vWF Protein, Human, Recombinant (His)

Von Willebrand Factor (VWF) is a multimeric glycoprotein involved in hemostasis in blood, binds receptors on the surface of platelets and in connective tissue, thereby mediating the adhesion of platelets to sites of vascular injury. From studies it appears that VWF protein uncoils under these circumstances, decelerating passing platelets. VWF protein is deficient or defective in von Willebrand disease (VWD) and is involved in a large number of other diseases, including thrombosis, thrombotic thrombocytopenic purpura, Stroke, Heyde's syndrome, possibly hemolytic-uremic syndrome and so on.

Von Willebrand Factor/vWF Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 103,500
200 μg	約5 days	￥ 183,500
500 μg	約5 days	￥ 603,500

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生物学的情報

Testing in progress

説明

Species	Human
Expression Host	CHO
Tag	His
Accession Number	AAB59458.1
別名	von Willebrand factor, VWD, F8VWF
Construction	A DNA sequence encoding the pro form of human von Willebrand factor (AAB59458.1) (Met1-Lys2813) was expressed with a C-terminal polyhistidine tag.
Protein Purity	≥ 75 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
分子量	308 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Von Willebrand Factor (VWF) is a multimeric glycoprotein involved in hemostasis in blood, binds receptors on the surface of platelets and in connective tissue, thereby mediating the adhesion of platelets to sites of vascular injury. From studies it appears that VWF protein uncoils under these circumstances, decelerating passing platelets. VWF protein is deficient or defective in von Willebrand disease (VWD) and is involved in a large number of other diseases, including thrombosis, thrombotic thrombocytopenic purpura, Stroke, Heyde's syndrome, possibly hemolytic-uremic syndrome and so on.

参考文献

1. Sadler JE. (1998) Biochemistry and genetics of von Willebrand factor. Annu Rev Biochem, 67: 395-424. 2. Batlle J,et al.(2009) Von Willebrand factor/factor VIII concentrates in the treatment of von Willebrand disease. Blood Coagul Fibrinolysis. 20(2):89-100. 3. Sadler JE. (2009) von Willebrand factor assembly and secretion. J Thromb Haemost. 7 Suppl 1:24-7. 4. Auton M,et al.(2010) The mechanism of VWF-mediated platelet GPIbalpha binding. Biophys J. 99(4):1192-201. 5. Bowen DJ. (2010) Sugar targets VWF for the chop. Blood. 115(13):2565. 6. Lpez JA,et al.(2010) VWF self-association: more bands for the buck. Blood. 116(19):3693-4.

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Von Willebrand Factor/vWF Protein, Human, Recombinant (His)

参考文献

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