VRK1 Protein, Human, Recombinant

VRK1 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 45.6 kDa and the accession number is Q99986.

VRK1 Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 114,500

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生物学的情報	Kinase activity untested
説明	VRK1 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 45.6 kDa and the accession number is Q99986.

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	Tag Free
Accession Number	Q99986
別名	PCH1, vaccinia related kinase 1, PCH1A
Construction	The Human VRK1 ( Q99986 ) (Met 1-Lys 396) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	45.6 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Research Background	VRK1 is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. Serine/threonine protein kinases are tumor suppressor that controls the activity of AMP-activated protein kinase family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. VRK1 contains 1 protein kinase domain and localizes to the nucleus. VRK1 gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. As a serine/threonine kinase, VRK1 phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1), also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy.

参考文献

1. Sugimoto J,et al. (1999) Isolation and mapping of a polymorphic CA repeat sequence at the human VRK1 locus. J Hum Genet. 44 (2): 133-4. 2. Lopez-Borges S,et al. (2000) The human vaccinia-related kinase 1 (VRK1) phosphorylates threonine-18 within the mdm-2 binding site of the p53 tumour suppressor protein. Oncogene. 19 (32): 3656-64. 3. Sevilla A,et al. (2004) c-Jun phosphorylation by the human vaccinia-related kinase 1 (VRK1) and its cooperation with the N-terminal kinase of c-Jun (JNK). Oncogene. 23 (55): 8950-8.

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VRK1 Protein, Human, Recombinant

参考文献

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