VSIG4 Protein, Cynomolgus, Recombinant (His)

VSIG4, a B7 family-related protein, is a negative regulator of T cell activation.T cell activation by APCs is positively and negatively regulated by members of the B7 family. Unlike that of B7 family members, surface expression of VSIG4 was restricted to resting tissue macrophages and absent upon activation by LPS or in autoimmune inflammatory foci. The specific expression of VSIG4 on resting macrophages in tissue suggests that this inhibitory ligand may be important for the maintenance of T cell unresponsiveness in healthy tissues.

VSIG4 Protein, Cynomolgus, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 112,000
500 μg	約5 days	￥ 447,500
1 mg	約5 days	￥ 746,000

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説明

Species	Cynomolgus
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	A0A7N9D9P6
別名	VSIG4, CRIg, Z39IG
Construction	Arg25-Ala288
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	30.16 kDa (predicted). Due to glycosylation, the protein migrates to 35-50 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	VSIG4, a B7 family-related protein, is a negative regulator of T cell activation.T cell activation by APCs is positively and negatively regulated by members of the B7 family. Unlike that of B7 family members, surface expression of VSIG4 was restricted to resting tissue macrophages and absent upon activation by LPS or in autoimmune inflammatory foci. The specific expression of VSIG4 on resting macrophages in tissue suggests that this inhibitory ligand may be important for the maintenance of T cell unresponsiveness in healthy tissues.

参考文献

1. Vogt L, et al. VSIG4, a B7 family-related protein, is a negative regulator of T cell activation. J Clin Invest. 2006 Oct;116(10):2817-26. doi: 10.1172/JCI25673. PMID: 17016562; PMCID: PMC1578631.

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VSIG4 Protein, Cynomolgus, Recombinant (His)

参考文献

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