VSIG4 Protein, Human, Recombinant (His)

VSIG4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 30.5 kDa and the accession number is Q9Y279-1.

VSIG4 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 88,500
200 μg	約5 days	￥ 156,500
500 μg	約5 days	￥ 330,500

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生物学的情報	Testing in progress
説明	VSIG4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 30.5 kDa and the accession number is Q9Y279-1.

Species	Human
Expression Host	HEK293 Cells
Tag	His
Accession Number	Q9Y279-1
別名	Z39IG, CRIg, V-set and immunoglobulin domain containing 4
Construction	The Human VSIG4 (Q9Y279-1) extracellular domain (Met 1-Pro 283) was expressed, fused with a polyhistidine tag at the C-terminus.
Protein Purity	≥ 96 % as determined by SDS-PAGE ≥ 95 % as determined by SEC-HPLC.
分子量	30.5 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	VSIG4 (V-set and immunoglobulin domain containing 4), also known as complement receptor of the immunoglobulin superfamily (CRIg) and Z39Ig, is a type I transmembrane glycoprotein. It is a B7 family-related protein and an Ig superfamily member. In contrast to the B7 family members which contain two IgG domains, VSIG4 contains one complete V-type I g domain and a truncated C-type I g domain. VSIG4 is exclusively expressed on tissue resident macrophages and binds to multimers of C3b and iC3b that are covalently attached to particle surfaces. No VSIG4 expression appears to be present in T and B cells. VSIG4 functions as a negative regulator of T cell activation, and may be involved in the maintenance of peripheral T cell tolerance, and is also identified as a potent suppressor of established inflammation. Mouse VSIG4 is synthesized as a 28 amino acid precursor that contains a signal sequence, a V-type I g domain (aa 36-115), one potential N-linked glycosylation site, and a single transmembrane domain. The V-type I g domain of mouse VSIG4 shares 86% and 8% aa sequence identity with the V-type I g domains of rat and human VSIG4, respectively.

参考文献

1. Vogt, L. et al., 2006, J Clin Invest.116: 2817-2826. 2. Helmy, K. et al., 2006, Cell. 124:915-927. 3. Wiesmann, C. et al., 2006, Nature. 444:217-220. 4. Zang,X. et al., 2006, J Clin Invest. 116: 2590-2593. 5. Katschke, KJ. et al., 2007, J. Exp. Med. 204:1319-1325. 6. He,JQ. et al., 2008, Mol Immunol. 45: 4041-4047.

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VSIG4 Protein, Human, Recombinant (His)

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