VTI1A Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 24.2 kDa and the accession number is Q96AJ9-2.
| 説明 |
VTI1A Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 24.2 kDa and the accession number is Q96AJ9-2. |
| Species |
Human
|
| Expression Host |
HEK293 Cells
|
| Tag |
N-His
|
| Accession Number |
Q96AJ9-2
|
| 別名 |
Vti1-rp2, MVti1, vesicle transport through interaction with t-SNAREs 1A, VTI1RP2, MMDS3
|
| Construction |
The Human VTI1A (Q96AJ9-2) (Met1-Arg187) was expressed with an N-terminal polyhistidine tag.
|
| Protein Purity |
> 95 % as determined by SDS-PAGE
|
| 分子量 |
24.2 kDa (predicted)
|
| Endotoxin |
< 1.0 EU/μg of the protein as determined by the LAL method.
|
| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
|
| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
|
| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
|
| Shipping |
In general, Lyophilized powders are shipping with blue ice.
|
| Research Background |
Evolutionarily conserved SNARE (soluble N-ethylmaleimide sensitive factor attachment protein receptors) proteins form a complex that drives membrane fusion in eukaryotes. VTI1A and VTI1B are the only members of their SNARE subclass and the yeast homolog Vti1p is essential for cell survival. VTI1A is involved in regulating insulin-stimulated trafficking of secretory vesicles enriched with both GLUT4 (glucose transporter) and Acrp30 in adipocytes; it also plays key roles in neuronal development and in selectively maintaining spontaneous neurotransmitter release.
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