Zika virus (ZIKV) (strain Zika SPH2015) E/Envelope protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 45.5 kDa and the accession number is ALU33341.1.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
100 μg | 在庫あり | ¥ 103,500 |
200 μg | 約5 days | ¥ 177,000 |
500 μg | 約5 days | ¥ 358,000 |
生物学的情報 | Testing in progress |
説明 | Zika virus (ZIKV) (strain Zika SPH2015) E/Envelope protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 45.5 kDa and the accession number is ALU33341.1. |
Species | ZIKV |
Expression Host | Baculovirus Insect Cells |
Tag | C-His |
Accession Number | ALU33341.1 |
Construction | The Zika virus (strain Zika SPH2015) E / Envelope (ALU33341.1) (Ile291-Gly694) was expressed with a polyhistidine tag at the C-terminus. |
Protein Purity |
> 90 % as determined by SDS-PAGE.
|
分子量 | 45.5 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Envelope of Zika virus is responsible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent with those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross-reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross-reactivity with dengue and YF.Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus. |
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