Powder: -20°C for 3 years | In solvent: -80°C for 1 year
1400W dihydrochloride (N-(3-(Aminomethyl)benzyl)acetamidine) is a highly effective and specific inhibitor of inducible NOS2 (iNOS).
説明 | 1400W dihydrochloride (N-(3-(Aminomethyl)benzyl)acetamidine) is a highly effective and specific inhibitor of inducible NOS2 (iNOS). |
ターゲット&IC50 | nNOS:2 μM(Ki), iNOS:<7 nM(Kd), eNOS:50 μM(Ki) |
In vitro | 1400W is either an irreversible inhibitor or an extremely slowly reversible inhibitor of human iNOS. Inhibition of human iNOS by 1400W is time-dependent. 1400W is competitive with L-arginine. 1400W is not a substrate for iNOS[1]. |
In vivo | 1400W selectively prevents microvasculature injury in rats. 1400W is greater than 50-fold more potent against iNOS than eNOS in a rat model of endotoxin-induced vascular injury. Moreover, 1400W also dose-dependently reduces LPS-induced vascular leakage associated with iNOS induction in the colon, lung, liver, kidney, and heart. The maximal protection is close to 100% for all organs except the kidney (kidney:54%)[1]. 1400W has an ameliorative effect on both oxidative and nitrosative stress in the kidneys against renal I/R injury in rats[2]. Treatment with 1400W can reduce the rate of growth of solid tumors in mice[4]. |
細胞研究 | RAW264.7 cells are treated with LPS/IFNγ for 16 h and GAPDH-p300 binding is abolished by the iNOS inhibitor 1400W (100 μM). Cell lysates are immunoprecipitated with an anti-p300 antibody and the immunoprecipitates are analysed by western blotting with an anti-GAPDH antibody. (Only for Reference) |
別名 | 1400W 2HCl, N-(3-(Aminomethyl)benzyl)acetamidine |
分子量 | 250.17 |
分子式 | C10H17Cl2N3 |
CAS No. | 214358-33-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 139.9 mM
DMSO: 55 mg/mL (219.85 mM)
You can also refer to dose conversion for different animals. 詳細
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1400W dihydrochloride 214358-33-5 Immunology/Inflammation NOS NO Synthase inhibit 1400W 2HCl N-(3-(Aminomethyl)benzyl)acetamidine Nitric oxide synthases 1400W Dihydrochloride 1400W Inhibitor inhibitor