15 Lipoxygenase 2 Protein, Human, Recombinant (His & GST)

15 Lipoxygenase 2 Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 103.7 kDa and the accession number is AAH35217.1.

15 Lipoxygenase 2 Protein, Human, Recombinant (His & GST)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 184,000

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説明	15 Lipoxygenase 2 Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 103.7 kDa and the accession number is AAH35217.1.

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	His, GST
Accession Number	AAH35217.1
別名	ALOX15B, arachidonate 15-lipoxygenase, type B, 15-LOX-2
Construction	The Human ALOX15B (AAH35217.1)( Met1-Ile676) was expressed the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	103.7 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM Nacl, 10% glycerol, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	ALOX15B is a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. ALOX15B converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. ALOX15B gene is located in a cluster of related genes and a pseudogene that spans approximately 1 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described.

参考文献

1. Kilty I. et al., 2000, Eur J Biochem. 266 (1): 83-93. 2. Sigal E. et al., 1990, J Biol Chem. 265 (9): 5113-20. 3. Brash AR. et al., 1997, Proc Natl Acad Sci. 94 (12): 6148-52.

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15 Lipoxygenase 2 Protein, Human, Recombinant (His & GST)

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