3CLpro/3C-like Protease Protein (P132H), SARS-COV-2, Recombinant

3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase. 3CLpro/3C-like Protease Protein (P132H), SARS-COV-2, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 33.8 kDa and the accession number is YP_009725301.1.

3CLpro/3C-like Protease Protein (P132H), SARS-COV-2, Recombinant

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 187,000
500 μg	約5 days	￥ 746,000
1 mg	約5 days	￥ 1,248,500

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説明

Species	SARS-CoV-2
Expression Host	E. coli
Tag	No Tag
Accession Number	YP_009725301.1
別名	3CL Protease, 3CL-Mpro, Mpro, M Proteinase
Construction	Recombinant SARS-CoV-2 3CLpro/3C-like Protease Protein (P132H) is expressed from E.coli without tag. It contains Ser1-Gln306(P132H).[Accession \|YP_009725301.1]
Protein Purity	> 95% as determined by Tris-Bis PAGE
分子量	The protein has a predicted MW of 33.8 kDa same as Tris-Bis PAGE result.

Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Supplied as 0.22μm filtered solution in 25mM HEPES, 2.5mM DTT, 10% Glycerol (pH 7.5).
Stability & Storage	Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase.

参考文献

1. Pillaiyar T, et al. An Overview of Severe Acute Respiratory Syndrome-Coronavirus (SARS-CoV) 3CL Protease Inhibitors: Peptidomimetics and Small Molecule Chemotherapy. J Med Chem. 2016 Jul 28;59(14):6595-628. doi: 10.1021/acs.jmedchem.5b0146Epub 2016 Feb 29. PMID: 26878082; PMCID: PMC7075655.

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3CLpro/3C-like Protease Protein (P132H), SARS-COV-2, Recombinant

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