Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. Alkaline phosphatase (Intestinal type) /ALPI Protein, Human, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-His-Flag tag. The predicted molecular weight is 54.5 kDa and the accession number is P09923.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
100 μg | 約5 days | ¥ 133,500 |
500 μg | 約5 days | ¥ 535,000 |
1 mg | 約5 days | ¥ 890,500 |
説明 | Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. Alkaline phosphatase (Intestinal type) /ALPI Protein, Human, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-His-Flag tag. The predicted molecular weight is 54.5 kDa and the accession number is P09923. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | N-His-Flag |
Accession Number | P09923 |
別名 | FLJ40094, ALPI, APTNAP, EC 3.1.3.1, AP-TNAP, IAP, TNAP, HOPS, FLJ93059, MGC161443, TNSALP, Akp2 |
Construction | Val20-Asp503 |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 54.5 kDa (predicted). Due to glycosylation, the protein migrates to 63-73 kDa based on Tris-Bis PAGE result. |
Endotoxin | < 1 EU/μg by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. |
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Please read the User Guide of Recombinant Proteins for more specific information.
Alkaline phosphatase (Intestinal type) /ALPI Protein, Human, Recombinant (His & Flag) FLJ40094 FLJ-40094 FLJ 93059 MGC 161443 ALPI FLJ 40094 MGC-161443 APTNAP EC 3.1.3.1 FLJ-93059 AP-TNAP IAP TNAP HOPS FLJ93059 MGC161443 TNSALP Akp2 recombinant recombinant-proteins proteins protein