B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. BAFF/TNFSF13B Protein, Human, Recombinant (monomeric hFc, Flag) is expressed in HEK293 mammalian cells with N-monomeric hFc and N-Flag tag. The predicted molecular weight is 74.9 kDa and the accession number is Q9Y275-1.
| 説明 |
B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. BAFF/TNFSF13B Protein, Human, Recombinant (monomeric hFc, Flag) is expressed in HEK293 mammalian cells with N-monomeric hFc and N-Flag tag. The predicted molecular weight is 74.9 kDa and the accession number is Q9Y275-1. |
| Species |
Human
|
| Expression Host |
HEK293 Cells
|
| Tag |
N-monomeric hFc, N-Flag
|
| Accession Number |
Q9Y275-1
|
| 別名 |
THANK, TALL1δ4 BAFF, TALL1Delta4 BAFF, TALL1, TNFSF20, TALL-1delta BAFF, DTL, BLyS, TNFSF13B, BAFF, TALL-1δ BAFF, TALL-1, CD257, ZTNF4
|
| Construction |
Thr141-Leu285
|
| Protein Purity |
> 95% as determined by Tris-Bis PAGE> 94% as determined by HPLC
|
| 分子量 |
74.9 kDa (predicted) same as Tris-Bis PAGE result.
|
| Endotoxin |
< 1 EU/μg by the LAL method.
|
| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
|
| Reconstitution |
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
|
| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
|
| Shipping |
In general, Lyophilized powders are shipping with blue ice.
|
| Research Background |
B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
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参考文献
Calculator
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Recombinant Proteins Dilute Calculator
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